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Please use this identifier to cite or link to this item: http://anchan.lib.ku.ac.th/agnet/handle/001/2156

ARN: TH201000002087
Authors: Payorm Cobelli
Varapong Chamarerk
Poonsak Mekwatanakarn
พยอม โคเบลลี่
วราพงษ์ ชมาฤกษ์
พูนศักดิ์ เมฆวัฒนากาญจน์
Authors' affiliation: Rice Department, Bangkok (Thailand). Bureau of Rice Research and Development. Ubon Ratchathani Rice Research Center
Rice Department, Bangkok (Thailand). Bureau of Rice Research and Development. Ubon Ratchathani Rice Research Center
Rice Department, Bangkok (Thailand). Bureau of Rice Research and Development. Ubon Ratchathani Rice Research Center
Authors' affiliation (Thai): กรมการข้าว สำนักวิจัยและพัฒนาข้าว ศูนย์วิจัยข้าวอุบลราชธานี
กรมการข้าว สำนักวิจัยและพัฒนาข้าว ศูนย์วิจัยข้าวอุบลราชธานี
กรมการข้าว สำนักวิจัยและพัฒนาข้าว ศูนย์วิจัยข้าวอุบลราชธานี
Title: การพัฒนาวิธีการสกัดดีเอ็นเอจากเมล็ดข้าวสารเพื่องานตรวจสอบความบริสุทธิ์ของพันธุ์ข้าวด้วยโมเลกุลเครื่องหมาย
Development of DNA extraction method from a single milled rice seed for rice varietal purity test by using molecular marker
Corporate authors (Thai): Rice Department, Bangkok (Thailand). Bureau of Rice Research and Development
Citation: [Proceedings of rice and temperate cereal crops annual conference 2008 volume 2/2], Bangkok (Thailand), p. 407-415
Format Extent: 228 p.
Issue Date: 2008
Language: Th
Keywords: Milled rice
DNA extraction method
Varietal purity test
Molecular marker
Subject Classification(ASC): F30
AGROVOC: Rice
Varieties
DNA
Extraction
Purity
Genetic markers
DNA fingerprinting
Abstract: Purity test of rice variety by using molecular marker or DNA fingerprinting is the most accurate method. Molecular marker technology could detect polymorphism between rice varieties. Milled rice seeds are not able to germinate due to loss of the embryo during milling process. The DNA extraction from rice leaves is not possible. Therefore, the varietal purity test requires extraction of DNA directly from a single milled rice seed. This method is difficult because the major composition of milled rice seeds is starch with low DNA content. In this study, we developed a very simple, rapid, convenient and economic feasible DNA extraction method from a single milled rice seed, which includes Proteinase K in SAD extraction buffer and 2x CTAB. Yield and quality of DNA extracted from a single milled rice seed was not different from those of leaf samples and also sufficient for DNA fingerprinting.
Format Extent: 228 หน้า
Availability : Rice Department, 50 Phahon Yothin Road, Chatuchak, Bangkok 10900, Thailand
Availability: กรมการข้าว
Appears in Collections:Rice Department

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