DSpace
 

Thai Agricultural Research Repository >
Research Institute >
Ministry of Agriculture and Cooperatives >
Rice Department >

Please use this identifier to cite or link to this item: http://anchan.lib.ku.ac.th/agnet/handle/001/2352

ARN: TH201000002283
Authors: Payorm Cobelli
Varapong Chamarerk
Poonsak Mekwatanakarn
Phikul Leelagud
Kanlaya Sansen
พยอม โคเบลลี่
วราพงษ์ ชมาฤกษ์
พูนศักดิ์ เมฆวัฒนากาญจน์
พิกุล ลีลากุด
กัลยา สานเสน
Authors' affiliation: Rice Department, Bangkok (Thailand). Bureau of Rice Research and Development. Ubon Ratchathani Rice Research Center
Rice Department, Bangkok (Thailand). Bureau of Rice Research and Development. Ubon Ratchathani Rice Research Center
Rice Department, Bangkok (Thailand). Bureau of Rice Research and Development. Ubon Ratchathani Rice Research Center
Rice Department, Bangkok (Thailand). Bureau of Rice Research and Development. Ubon Ratchathani Rice Research Center
Rice Department, Bangkok (Thailand). Bureau of Rice Research and Development. Ubon Ratchathani Rice Research Center
Authors' affiliation (Thai): กรมการข้าว สำนักวิจัยและพัฒนาข้าว ศูนย์วิจัยข้าวอุบลราชธานี
กรมการข้าว สำนักวิจัยและพัฒนาข้าว ศูนย์วิจัยข้าวอุบลราชธานี
กรมการข้าว สำนักวิจัยและพัฒนาข้าว ศูนย์วิจัยข้าวอุบลราชธานี
กรมการข้าว สำนักวิจัยและพัฒนาข้าว ศูนย์วิจัยข้าวอุบลราชธานี
กรมการข้าว สำนักวิจัยและพัฒนาข้าว ศูนย์วิจัยข้าวอุบลราชธานี
Title: การพัฒนาวิธีการตรวจสอบความบริสุทธิ์ของพันธุ์ข้าวด้วยโมเลกุลเครื่องหมาย
Development of method for rice varietal purity test by using molecular markers
Corporate authors (Thai): Rice Department, Bangkok (Thailand). Bureau of Rice Research and Development
Citation: [Proceedings of rice and temperate cereal crops annual conference 2009], Bangkok (Thailand), p. 166-186
Format Extent: 389 p.
Issue Date: 2009
Language: Th
Keywords: Rice
Varietal purity test
DNA fingerprinting
Molecular markers
Allele standard
Milled Rice
Subject Classification(ASC): F30
AGROVOC: Oryza sativa
Varieties
Pure lines
Genetic markers
Genes
DNA fingerprinting
Abstract: Purity test of rice varietiesy by using molecular markers or DNA fingerprintings is the most accurate method. Molecular marker technology could detect polymorphism between rice varieties. Milled rice seeds are not able to germinate due to loss of the embryo during milling process. The DNA extraction from rice leaves is not possible. Therefore, the varietal purity test requires extraction of DNA directly from a single milled rice seed. This method is difficult because the major composition of milled rice seeds is starch with low DNA content. In this study, we developed a very simple, rapid, convenient and economically feasible DNA extraction method by uang from a single milled rice seed, by comparing three different methods, (1) extraction method by using proteinase K in SDS extraction buffer and 2x CTAB method, (2) extraction method by using a mobile automated DNA extractor (Maxwell) and (3) extraction method by using an automated DNA extractor (Xiril). Yield and quality of DNA extracted from a single milled rice seed was not different from those of leaf samples and also sufficient for DNA fingerprinting. In addition, we had surveyed and analyzed 32 SSR markers for rice allele standard. Twenty-three SSR markers were analyzed with cluster analysis and the results showed 8 groups of makers that can distinguish the rice variety KDML105 from CNT1 variety. Thirteen SSR markers were classified using cluster analysis and revealed 4 groups of makers that can distinguish the rice variety KDML105 from PTT1 variety. All these SSR markers showed 4-13 alleles on 27 rice lines/varieties.
Format Extent: 389 หน้า
Availability : Rice Department, 50 Phahon Yothin Road, Chatuchak, Bangkok 10900, Thailand
Availability: กรมการข้าว
Appears in Collections:Rice Department

Files in This Item:

File Description SizeFormat
RIC020344c.pdf1.19 MBAdobe PDFView/Open
RIC020344b.pdf98.39 kBAdobe PDFView/Open
RIC020344a.pdf5.52 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2009  The DSpace Foundation - Feedback