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Please use this identifier to cite or link to this item: http://anchan.lib.ku.ac.th/agnet/handle/001/4662

ARN: TH201200004582
Authors: Niran Juntawong
Prasart Kuermanee
นิรันดร์ จันทวงศ์
ประศาสตร์ เกื้อมณี
Authors' affiliation: Kasetsart University, Bangkok (Thailand). Faculty of Science. Department of Botany
Kasetsart University, Bangkok (Thailand). Faculty of Science. Department of Botany
Authors' affiliation (Thai): มหาวิทยาลัยเกษตรศาสตร์ คณะวิทยาศาสตร์ ภาควิชาพฤกษศาสตร์
มหาวิทยาลัยเกษตรศาสตร์ คณะวิทยาศาสตร์ ภาควิชาพฤกษศาสตร์
Title: การโคลนนิ่งและหาลำดับเบสของยีนที่ตอบสนองต่อสภาวะแล้งและความสัมพันธ์ระหว่างลักษณะโครงสร้างทางกายวิภาคกับการทนทานต่อสภาพแล้งในข้าวโพด (Zea mays L.)
Cloning and sequencing of drought responsible gene and relationship between anatomical structure and drought tolerance in maize (Zea mays L.)
Corporate authors (Thai): Kasetsart University, Bangkok (Thailand)
Citation: Proceedings of the 4th Workshop of Corn and Sorghum Research Project of Kasetsart University: Corn and sorghum yield increasing to improve the quality of life and environmental sustainability, Bangkok (Thailand), p. 55-71
Issue Date: 2010
Language: tha
Keywords: Zea mays
Cloning
Sequencing
Drought responsible gene
Anatomical structure
Drought tolerance
9-cis epoxycarotenoid dioxygenase
VP14
NCED
Subject Classification(ASC): F30
H50
F50
AGROVOC: Zea mays
Varieties
Genes
Drought resistance
Cloning
Nucleotide sequence
Plant anatomy
ABA
Zea mays
พันธุ์
ยีน
ความต้านทานสภาพแล้ง
โคลนนิ่ง
การเรียงตัวของนิวคลีโอไทด์
กายวิภาคของพืช
เอบีเอ
Abstract: Drought stress could induce expressions of various genes that are involved in stress tolerance and responses. Recent studies were emphasized on a gene encoded for the 9-cis epoxycarotenoid dioxygenase (NCED) enzyme which catalyzes the biosynthesis of abscisic acid (ABA) in plants. However, little is known about drought tolerance genes in maize. We designed primers from the VP14 sequence in the NCBI gene bank for the study of expression of drought tolerance gene in SW 2301 drought tolerant and KSX 4605 drought sensitive lines. The RT-PCR showed an expression of drought responsible gene in SW 2301 drought tolerant line. Under drought condition, the expression of the responsible gene was higher in stem apical meristem than in root and leaf tissues. The cDNA of this gene was cloned by rapid amplification of cDNA ends (RACE). The full-length sequence of this drought responsible gene was 1607 bp with the open reading frame at 56-1345 bp. Anatomical characteristics relating to water utilization including stomata, bulliform cell, vascular bundle and vessel were investigated in 5 varieties of corn. It was found that the drought tolerant variety had a small number of stomata, a large number of vascular bundle and a large size of vessel. Among the five varieties (Ki 3, Ki 11, Ki 20, Suwan 2301 and Insee), Ki 11 variety showed the smallest number of stomata, the largest number of vascular bundle and the largest size of vessel. Overall of this study, it can be concluded that Ki 11 is the best drought tolerant variety followed by Ki 20 variety whilst Ki 3 variety showed the most susceptible to drought.
Availability : National Corn and Sorghum Research Center, Nakhon Ratchasima (Thailand)
Availability: ศูนย์วิจัยข้าวโพดและข้าวฟ่างแห่งชาติ
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